Skip to main content
Addgene

GFP KNL1 wt hardened and RVSF->AAAA
(Plasmid #45225)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45225 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    unknown

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP KNL1 with RVSF-AAAA mutation, resistant to KNL1 siRNA
  • Alt name
    CASC5
  • Alt name
    pDL019
  • Species
    H. sapiens (human)
  • Mutation
    RVSF mutated to AAAA
  • GenBank ID
  • Entrez Gene
    KNL1 (a.k.a. AF15Q14, CASC5, CT29, D40, MCPH4, PPP1R55, Spc7, hKNL-1, hSpc105)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP KNL1 wt hardened and RVSF->AAAA was a gift from Iain Cheeseman & Michael Lampson (Addgene plasmid # 45225 ; http://n2t.net/addgene:45225 ; RRID:Addgene_45225)

  • For your References section:

    Regulated targeting of protein phosphatase 1 to the outer kinetochore by KNL1 opposes Aurora B kinase. Liu D, Vleugel M, Backer CB, Hori T, Fukagawa T, Cheeseman IM, Lampson MA. J Cell Biol. 2010 Mar 22;188(6):809-20. doi: 10.1083/jcb.201001006. Epub 2010 Mar 15. 10.1083/jcb.201001006 PubMed 20231380
Related items:
Commonly requested with: