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Addgene

GFP KNL1 wt hardened
(Plasmid #45223)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45223 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP KNL1 wt hardened (mutated KNL1 full length that is resisted to KNL1 siRNA)
  • Alt name
    CASC5
  • Alt name
    pDL017
  • Species
    H. sapiens (human)
  • Mutation
    E172G
  • Entrez Gene
    KNL1 (a.k.a. AF15Q14, CASC5, CT29, D40, MCPH4, PPP1R55, Spc7, hKNL-1, hSpc105)
  • Tag / Fusion Protein
    • TEV S15 (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP KNL1 wt hardened was a gift from Michael Lampson (Addgene plasmid # 45223 ; http://n2t.net/addgene:45223 ; RRID:Addgene_45223)

  • For your References section:

    Regulated targeting of protein phosphatase 1 to the outer kinetochore by KNL1 opposes Aurora B kinase. Liu D, Vleugel M, Backer CB, Hori T, Fukagawa T, Cheeseman IM, Lampson MA. J Cell Biol. 2010 Mar 22;188(6):809-20. doi: 10.1083/jcb.201001006. Epub 2010 Mar 15. 10.1083/jcb.201001006 PubMed 20231380
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