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CENP-B mCherry PP1 gamma
(Plasmid #45221)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45221 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CENP-B mCherry PP1 gamma
  • Alt name
    pKL025
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer not available
  • 3′ sequencing primer not available
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CENP-B mCherry PP1 gamma was a gift from Iain Cheeseman & Michael Lampson (Addgene plasmid # 45221 ; http://n2t.net/addgene:45221 ; RRID:Addgene_45221)

  • For your References section:

    Regulated targeting of protein phosphatase 1 to the outer kinetochore by KNL1 opposes Aurora B kinase. Liu D, Vleugel M, Backer CB, Hori T, Fukagawa T, Cheeseman IM, Lampson MA. J Cell Biol. 2010 Mar 22;188(6):809-20. doi: 10.1083/jcb.201001006. Epub 2010 Mar 15. 10.1083/jcb.201001006 PubMed 20231380
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