untargeted Plk1 FRET sensor Myt1 substrate
(Plasmid
#45217)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45217 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Total vector size (bp) 7347
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameuntargeted Plk1 FRET sensor Myt1 substrate
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Alt namepML065
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SpeciesH. sapiens (human)
- Promoter t7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site n/a (not destroyed)
- 3′ cloning site n/a (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
untargeted Plk1 FRET sensor Myt1 substrate was a gift from Michael Lampson (Addgene plasmid # 45217 ; http://n2t.net/addgene:45217 ; RRID:Addgene_45217) -
For your References section:
Midzone activation of aurora B in anaphase produces an intracellular phosphorylation gradient. Fuller BG, Lampson MA, Foley EA, Rosasco-Nitcher S, Le KV, Tobelmann P, Brautigan DL, Stukenberg PT, Kapoor TM. Nature. 2008 Jun 19;453(7198):1132-6. doi: 10.1038/nature06923. Epub 2008 May 7. 10.1038/nature06923 PubMed 18463638