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Addgene

Histone H2B targeted Aurora B FRET sensor Kif2 substrate
(Plasmid #45216)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45216 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2398
  • Total vector size (bp) 7753
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Histone H2B-targeted Aurora B FRET sensor
  • Alt name
    pML049
  • Insert Size (bp)
    2398
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer Not available
  • 3′ sequencing primer Not available
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Histone H2B targeted Aurora B FRET sensor Kif2 substrate was a gift from Michael Lampson (Addgene plasmid # 45216 ; http://n2t.net/addgene:45216 ; RRID:Addgene_45216)

  • For your References section:

    Midzone activation of aurora B in anaphase produces an intracellular phosphorylation gradient. Fuller BG, Lampson MA, Foley EA, Rosasco-Nitcher S, Le KV, Tobelmann P, Brautigan DL, Stukenberg PT, Kapoor TM. Nature. 2008 Jun 19;453(7198):1132-6. doi: 10.1038/nature06923. Epub 2008 May 7. 10.1038/nature06923 PubMed 18463638
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