Skip to main content
Addgene

Hec1 mCherry Plk1 K82R
(Plasmid #45206)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45206 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5355
  • Total vector size (bp) 9865
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hec1-targeted mCherry Plk1 K82R
  • Alt name
    pDL032
  • Insert Size (bp)
    9865
  • Mutation
    Plk1 K82R mutation
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer not available
  • 3′ sequencing primer not available
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Hec1 mCherry Plk1 K82R was a gift from Michael Lampson (Addgene plasmid # 45206 ; http://n2t.net/addgene:45206 ; RRID:Addgene_45206)

  • For your References section:

    Polo-like kinase-1 regulates kinetochore-microtubule dynamics and spindle checkpoint silencing. Liu D, Davydenko O, Lampson MA. J Cell Biol. 2012 Aug 20;198(4):491-9. doi: 10.1083/jcb.201205090. 10.1083/jcb.201205090 PubMed 22908307
Related items:
Commonly requested with: