Hec1 mCherry Plk1 T210D
(Plasmid
#45205)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45205 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5348
- Total vector size (bp) 9865
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHec1-targeted mCherry Plk1 T210D
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Alt namepDL031
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Insert Size (bp)4510
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MutationPlk1 T210D mutation
- Promoter T7
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Tag
/ Fusion Protein
- mCherry
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer not available
- 3′ sequencing primer not available (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Hec1 mCherry Plk1 T210D was a gift from Michael Lampson (Addgene plasmid # 45205 ; http://n2t.net/addgene:45205 ; RRID:Addgene_45205) -
For your References section:
Polo-like kinase-1 regulates kinetochore-microtubule dynamics and spindle checkpoint silencing. Liu D, Davydenko O, Lampson MA. J Cell Biol. 2012 Aug 20;198(4):491-9. doi: 10.1083/jcb.201205090. 10.1083/jcb.201205090 PubMed 22908307
