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Addgene

Plk1 FRET sensor c-jun substrate
(Plasmid #45203)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45203 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Total vector size (bp) 4370
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Plk1 FRET sensor with c-jun substrate
  • Alt name
    untargeted Plk1 FRET sensor
  • Alt name
    pOD004
  • Promoter T7
  • Tag / Fusion Protein
    • CyPET, YPET

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Plk1 FRET sensor c-jun substrate was a gift from Michael Lampson (Addgene plasmid # 45203 ; http://n2t.net/addgene:45203 ; RRID:Addgene_45203)

  • For your References section:

    Polo-like kinase-1 regulates kinetochore-microtubule dynamics and spindle checkpoint silencing. Liu D, Davydenko O, Lampson MA. J Cell Biol. 2012 Aug 20;198(4):491-9. doi: 10.1083/jcb.201205090. 10.1083/jcb.201205090 PubMed 22908307
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