H2A-Bbd-GFP
(Plasmid
#45173)
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45173 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1-CT-GFP
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 6141
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH2AFB1
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Alt nameH2A-Bbd
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Alt nameH2A.B
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SpeciesH. sapiens (human)
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Insert Size (bp)360
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GenBank IDAF254576
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Entrez GeneH2AFB1 (a.k.a. H2A.B, H2A.Bbd)
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer GFP-N (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
H2A-Bbd-GFP was a gift from Brian Chadwick & Hunt Willard (Addgene plasmid # 45173 ; http://n2t.net/addgene:45173 ; RRID:Addgene_45173) -
For your References section:
A novel chromatin protein, distantly related to histone H2A, is largely excluded from the inactive X chromosome. Chadwick BP, Willard HF. J Cell Biol. 2001 Jan 22;152(2):375-84. 10.1083/jcb.152.2.375 PubMed 11266453