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Addgene

H2A-Bbd-MYC
(Plasmid #45172)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45172 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1-CT-MYC-A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2AFB1
  • Alt name
    H2A-Bbd
  • Alt name
    H2A.B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    360
  • GenBank ID
    AF254576
  • Entrez Gene
    H2AFB1 (a.k.a. H2A.B, H2A.Bbd)
  • Tags / Fusion Proteins
    • MYC (C terminal on backbone)
    • HIS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-REV
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H2A-Bbd-MYC was a gift from Brian Chadwick & Hunt Willard (Addgene plasmid # 45172 ; http://n2t.net/addgene:45172 ; RRID:Addgene_45172)

  • For your References section:

    A novel chromatin protein, distantly related to histone H2A, is largely excluded from the inactive X chromosome. Chadwick BP, Willard HF. J Cell Biol. 2001 Jan 22;152(2):375-84. 10.1083/jcb.152.2.375 PubMed 11266453
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