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Addgene

mH2A2-CT-MYC
(Plasmid #45170)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45170 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1-CT-MYC-HIS-B
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2Afy2
  • Alt name
    mH2A2
  • Alt name
    macroH2A2
  • Alt name
    macroH2A2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1100
  • GenBank ID
    AF336304
  • Entrez Gene
    MACROH2A2 (a.k.a. H2AFY2)
  • Entrez Gene
    H2AFY2 (a.k.a. MACROH2A2)
  • Tags / Fusion Proteins
    • MYC (C terminal on backbone)
    • HIS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-REV
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing result identified a single nucleotide mismatch at bp# 653 when compared to GenBank ID AF336304. This silent mutation does not affect the protein sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mH2A2-CT-MYC was a gift from Brian Chadwick & Hunt Willard (Addgene plasmid # 45170 ; http://n2t.net/addgene:45170 ; RRID:Addgene_45170)
  • For your References section:

    Histone H2A variants and the inactive X chromosome: identification of a second macroH2A variant. Chadwick BP, Willard HF. Hum Mol Genet. 2001 May 1;10(10):1101-13. 10.1093/hmg/10.10.1101 PubMed 11331621