mH2A2-CT-MYC
(Plasmid
#45170)
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45170 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1-CT-MYC-HIS-B
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH2Afy2
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Alt namemH2A2
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Alt namemacroH2A2
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Alt namemacroH2A2
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SpeciesH. sapiens (human)
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Insert Size (bp)1100
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GenBank IDAF336304
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Entrez GeneMACROH2A2 (a.k.a. H2AFY2)
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Entrez GeneH2AFY2 (a.k.a. MACROH2A2)
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Tags
/ Fusion Proteins
- MYC (C terminal on backbone)
- HIS (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH-REV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that Addgene's sequencing result identified a single nucleotide mismatch at bp# 653 when compared to GenBank ID AF336304. This silent mutation does not affect the protein sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mH2A2-CT-MYC was a gift from Brian Chadwick & Hunt Willard (Addgene plasmid # 45170 ; http://n2t.net/addgene:45170 ; RRID:Addgene_45170) -
For your References section:
Histone H2A variants and the inactive X chromosome: identification of a second macroH2A variant. Chadwick BP, Willard HF. Hum Mol Genet. 2001 May 1;10(10):1101-13. 10.1093/hmg/10.10.1101 PubMed 11331621