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Addgene

mH2A1.2-CT-GFP
(Plasmid #45169)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45169 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1-CT-GFP
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2Afy
  • Alt name
    mH2A1.2
  • Alt name
    macroH2A1.2
  • Alt name
    macroH2A1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1100
  • GenBank ID
    NP_004884.1 / NP_001035248
  • Entrez Gene
    MACROH2A1 (a.k.a. H2A.y, H2A/y, H2AF12M, H2AFY, MACROH2A1.1, mH2A1, macroH2A1.2)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer GFP-N
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mH2A1.2-CT-GFP was a gift from Brian Chadwick & Hunt Willard (Addgene plasmid # 45169 ; http://n2t.net/addgene:45169 ; RRID:Addgene_45169)

  • For your References section:

    Histone H2A variants and the inactive X chromosome: identification of a second macroH2A variant. Chadwick BP, Willard HF. Hum Mol Genet. 2001 May 1;10(10):1101-13. 10.1093/hmg/10.10.1101 PubMed 11331621
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