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pcDNA3-Drp1K38A
(Plasmid #45161)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45161 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 7521
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Drp1
  • Alt name
    Dynamin related protein 1
  • Alt name
    DNM1L
  • Alt name
    DLP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2100
  • Mutation
    K38A
  • GenBank ID
    NM_005690
  • Entrez Gene
    DNM1L (a.k.a. DLP1, DRP1, DVLP, DYMPLE, EMPF, EMPF1, HDYNIV, OPA5)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (AYPYDVPDY) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis.
Frank S, Gaume B, Bergmann-Leitner ES, Leitner WW, Robert EG, Catez F, Smith CL, Youle RJ
In Dev Cell. 2001 Oct;1(4):515-25.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Drp1K38A was a gift from Alexander van der Bliek & Richard Youle (Addgene plasmid # 45161 ; http://n2t.net/addgene:45161 ; RRID:Addgene_45161)

  • For your References section:

    A human dynamin-related protein controls the distribution of mitochondria. Smirnova E, Shurland DL, Ryazantsev SN, van der Bliek AM. J Cell Biol. 1998 Oct 19;143(2):351-8. 10.1083/jcb.143.2.351 PubMed 9786947
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