PLlacO-1-mfLon
(Plasmid #45152)

• Depositing Lab: Michel Maharbiz
• Publication: Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

Backbone

• Vector backbone: pBbC6c-RFP
• Backbone manufacturer: Keasling Lab
• Backbone size w/o insert (bp): 3489
• Total vector size (bp): 5883
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lon
• Species: Mesoplasma florum
• Insert Size (bp): 2400
• Promoter: PLlacO-1
• Tag / Fusion Protein:
  • ssRA degradation (LVA) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CCACGCCGAAACAAG
• 3′ sequencing primer: CGAAGGTGAGCCAGTGT

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Was obtained from Addgene http://www.addgene.org/21867/

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry