Cre Stoplight
(Plasmid #45150)

• Depositing Lab: Thomas Hughes
• Publication: Yang et al Biotechniques. 2001 Nov;31(5):1036, 1038, 1040-1.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogene
• Backbone size w/o insert (bp): 3342
• Total vector size (bp): 5387
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cre Stoplight
• Alt name: Original Stoplight
• Insert Size (bp): 2045
• Promoter: CMV
• Tags / Fusion Proteins:
  • DsRed (N terminal on insert)
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: refer to map (unknown if destroyed)
• 3′ cloning site: refer to map (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: M13 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry