FRas2-M Acceptor
(Plasmid
#45149)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45149 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCI
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 5640
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemRFP - Raf binding domain-mRFP
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Alt namemRFP
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Alt nameRaf Binding Domain
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Insert Size (bp)1650
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Mutationlysine 65 changed to glutamine, lysine 108 mutated to alanine in raf binding domain
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Tag
/ Fusion Protein
- mRFP
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FRas2-M Acceptor was a gift from Ryohei Yasuda (Addgene plasmid # 45149 ; http://n2t.net/addgene:45149 ; RRID:Addgene_45149) -
For your References section:
An improved Ras sensor for highly sensitive and quantitative FRET-FLIM imaging. Oliveira AF, Yasuda R. PLoS One. 2013;8(1):e52874. doi: 10.1371/journal.pone.0052874. Epub 2013 Jan 14. 10.1371/journal.pone.0052874 PubMed 23349692
Map uploaded by the depositor.
