FRas2-F Acceptor
(Plasmid #45148)

• Depositing Lab: Ryohei Yasuda
• Publication: Oliveira et al PLoS One. 2013;8(1):e52874. doi: 10.1371/journal.pone.0052874. Epub 2013 Jan 14.

Backbone

• Vector backbone: pCI
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 5640
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mRFP - Raf binding domain-mRFP
• Alt name: monomeric Red Fluorescent Protein
• Alt name: Raf Binding Domain
• Insert Size (bp): 1650

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that the XhoI site in the depositor's assembled sequence is not actually present in the plasmid (see Addgene's QC sequence with CMV-F for this region).