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Addgene

FRas2-F Acceptor
(Plasmid #45148)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45148 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 5640
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mRFP - Raf binding domain-mRFP
  • Alt name
    monomeric Red Fluorescent Protein
  • Alt name
    Raf Binding Domain
  • Insert Size (bp)
    1650

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that the XhoI site in the depositor's assembled sequence is not actually present in the plasmid (see Addgene's QC sequence with CMV-F for this region).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FRas2-F Acceptor was a gift from Ryohei Yasuda (Addgene plasmid # 45148 ; http://n2t.net/addgene:45148 ; RRID:Addgene_45148)

  • For your References section:

    An improved Ras sensor for highly sensitive and quantitative FRET-FLIM imaging. Oliveira AF, Yasuda R. PLoS One. 2013;8(1):e52874. doi: 10.1371/journal.pone.0052874. Epub 2013 Jan 14. 10.1371/journal.pone.0052874 PubMed 23349692
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