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Addgene

pKG215
(Plasmid #45110)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45110 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIC242
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP-Laci_NLS
  • Species
    H. sapiens (human)
  • Tags / Fusion Proteins
    • GFP (N terminal on insert)
    • tev (N terminal on insert)
    • s peptide (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKG215 was a gift from Iain Cheeseman (Addgene plasmid # 45110 ; http://n2t.net/addgene:45110 ; RRID:Addgene_45110)

  • For your References section:

    Induced ectopic kinetochore assembly bypasses the requirement for CENP-A nucleosomes. Gascoigne KE, Takeuchi K, Suzuki A, Hori T, Fukagawa T, Cheeseman IM. Cell. 2011 Apr 29;145(3):410-22. doi: 10.1016/j.cell.2011.03.031. 10.1016/j.cell.2011.03.031 PubMed 21529714
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