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              Depositing Lab
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          Sequence Information
 
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45110 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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            Vector backbonepIC242
 - Backbone size w/o insert (bp) 6000
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              Vector typeMammalian Expression
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                Selectable markersBlasticidin
 
Growth in Bacteria
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            Bacterial Resistance(s)Ampicillin, 100 μg/mL
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            Growth Temperature37°C
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            Growth Strain(s)DH5alpha
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            Copy numberUnknown
 
Gene/Insert
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                Gene/Insert nameGFP-Laci_NLS
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                    SpeciesH. sapiens (human)
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        Tags
        / Fusion Proteins
    
- GFP (N terminal on insert)
 - tev (N terminal on insert)
 - s peptide (N terminal on insert)
 
 
Resource Information
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            Article Citing this Plasmid
 
Terms and Licenses
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        Academic/Nonprofit Terms
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      Industry Terms
- Not Available to Industry
 
 
Trademarks:
- Zeocin® is an InvivoGen trademark.
 
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKG215 was a gift from Iain Cheeseman (Addgene plasmid # 45110 ; http://n2t.net/addgene:45110 ; RRID:Addgene_45110) - 
                
For your References section:
Induced ectopic kinetochore assembly bypasses the requirement for CENP-A nucleosomes. Gascoigne KE, Takeuchi K, Suzuki A, Hori T, Fukagawa T, Cheeseman IM. Cell. 2011 Apr 29;145(3):410-22. doi: 10.1016/j.cell.2011.03.031. 10.1016/j.cell.2011.03.031 PubMed 21529714