pSO221 Phis-72 BirA GFP
(Plasmid #45100)

• Depositing Lab: Steven Henikoff
• Publication: Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi: 10.1101/gr.131748.111. Epub 2012 Jan 4.

Backbone

• Vector backbone: pWormgate2
• Backbone size w/o insert (bp): 2884
• Total vector size (bp): 12471
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: BirA::GFP
• Species: E. coli
• Insert Size (bp): 1844
• GenBank ID: M10123.1
• Promoter: his-72
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: ??
• 3′ sequencing primer: GFP-R

Gene/Insert 2

• Gene/Insert name: unc-119
• Species: C. elegans (nematode)
• Insert Size (bp): 5708
• Entrez Gene: unc-119 (a.k.a. CELE_M142.1)
• Promoter: unc-119

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: ??

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry