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pCI-neo:Beta2meGFP
(Plasmid #45096)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45096 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5472
  • Total vector size (bp) 7707
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nAChR beta2
  • Alt name
    beta2-mGFP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2235
  • Mutation
    There are strain differences between C57BJ/6 and 129 in Chrnb2 at position 380. In C57BJ/6 it is a glutamate and in 129 it is a lysine. All of the forms of β2 currently in the lab are of the 129 variant except the β2 with GFP which was changed to a glutamate when GFP was inserted.
  • Entrez Gene
    Chrnb2 (a.k.a. Acrb-2, Acrb2, C030030P04Rik, [b]2-nAchR)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    J. Stizel (University of Colorado, Boulder, CO)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Pi ; Henry A. Lester

β2 was cloned into pCI-neo at the restriction site EcoRI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-neo:Beta2meGFP was a gift from Henry Lester (Addgene plasmid # 45096 ; http://n2t.net/addgene:45096 ; RRID:Addgene_45096)

  • For your References section:

    Nicotine up-regulates alpha4beta2 nicotinic receptors and ER exit sites via stoichiometry-dependent chaperoning. Srinivasan R, Pantoja R, Moss FJ, Mackey ED, Son CD, Miwa J, Lester HA. J Gen Physiol. 2011 Jan;137(1):59-79. 10.1085/jgp.201010532 PubMed 21187334
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