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Addgene

pBS-gH4
(Plasmid #45081)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45081 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II SK(+)
  • Backbone manufacturer
    Agilent Technologies
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 3900
  • Vector type
    RNAi ; in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    germinal Histone H4
  • Alt name
    gH4
  • Alt name
    Histone H4
  • Alt name
    PL04010A1G07
  • Species
    Schmidtea mediterranea
  • Insert Size (bp)
    922
  • Promoter T7 sense, T3 antisense

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer M13 –20
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-gH4 was a gift from Phillip Newmark (Addgene plasmid # 45081 ; http://n2t.net/addgene:45081 ; RRID:Addgene_45081)

  • For your References section:

    PIWI homologs mediate histone H4 mRNA localization to planarian chromatoid bodies. Rouhana L, Weiss JA, King RS, Newmark PA. Development. 2014 Jul;141(13):2592-601. doi: 10.1242/dev.101618. Epub 2014 Jun 5. 10.1242/dev.101618 PubMed 24903754
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