pCI-neo:alpha4-mcherry
(Plasmid
#45073)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45073 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCI-neo
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5472
- Total vector size (bp) 8069
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namealpha4-mcherry
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Alt namenAChR alpha4-mcherry
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SpeciesM. musculus (mouse)
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Insert Size (bp)2597
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Entrez GeneChrna4 (a.k.a. Acra-4, Acra4, EBN1, ENFL1)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJ.Stizel (University of Colorado, Boulder, CO)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Principal Investigator Henry A. Lester
α4 cloned into EcoRI sites of pCI-neo
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-neo:alpha4-mcherry was a gift from Henry Lester (Addgene plasmid # 45073 ; http://n2t.net/addgene:45073 ; RRID:Addgene_45073) -
For your References section:
Nicotine up-regulates alpha4beta2 nicotinic receptors and ER exit sites via stoichiometry-dependent chaperoning. Srinivasan R, Pantoja R, Moss FJ, Mackey ED, Son CD, Miwa J, Lester HA. J Gen Physiol. 2011 Jan;137(1):59-79. 10.1085/jgp.201010532 PubMed 21187334