Lmx1a-in situ probe
(Plasmid
#45071)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45071 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCRII-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3950
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLmx1a LIM homeobox transcription factor 1 alpha (residues 94-1149)
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Alt nameLmx1a
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SpeciesM. musculus (mouse)
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Insert Size (bp)1150
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MutationLmx1a residues 94-1149
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GenBank IDAF226662
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Entrez GeneLmx1a (a.k.a. Lmx1.1, dr, dreher, sst)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For antisense RNA: cut with EcoRV, use SP6. For sense RNA: cut with BamHI, use T7.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lmx1a-in situ probe was a gift from Kathleen Millen (Addgene plasmid # 45071 ; http://n2t.net/addgene:45071 ; RRID:Addgene_45071)
Map uploaded by the depositor.