pCIG-Lmx1a
(Plasmid
#45070)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45070 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCIG
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Backbone manufacturerAndrew McMahon lab
- Backbone size w/o insert (bp) 6240
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLmx1a LIM homeobox transcription factor 1 alpha
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Alt nameLmx1a
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SpeciesM. musculus (mouse)
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Insert Size (bp)1150
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GenBank IDAF226662
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Entrez GeneLmx1a (a.k.a. Lmx1.1, dr, dreher, sst)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xhol (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer IRES-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid produces mouse Lmx1a.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCIG-Lmx1a was a gift from Kathleen Millen (Addgene plasmid # 45070 ; http://n2t.net/addgene:45070 ; RRID:Addgene_45070)
Map uploaded by the depositor.