Skip to main content
Addgene

UAS-dar1-V5
(Plasmid #45056)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45056 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pUAST
  • Backbone size w/o insert (bp) 8900
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dar1
  • Alt name
    CG12029
  • Alt name
    dendritic arbor reduction 1
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    dar1 (a.k.a. Dmel_CG12029, CG12029, Dar1, Dmel\CG12029, l(3)63Ed, l(3)SH5)
  • Promoter HS promoter
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pCasper-hs
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CG12029 cDNA was tagged with the V5 tag and subcloned into pUAST.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UAS-dar1-V5 was a gift from Bing Ye (Addgene plasmid # 45056 ; http://n2t.net/addgene:45056 ; RRID:Addgene_45056)

  • For your References section:

    Differential regulation of dendritic and axonal development by the novel Kruppel-like factor Dar1. Ye B, Kim JH, Yang L, McLachlan I, Younger S, Jan LY, Jan YN. J Neurosci. 2011 Mar 2;31(9):3309-19. doi: 10.1523/JNEUROSCI.6307-10.2011. 10.1523/JNEUROSCI.6307-10.2011 PubMed 21368042
Related items: