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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45050 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYX142
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemtGFP
- Promoter TPI
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer none
- 3′ sequencing primer none (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
additional information can be found at:
http://www.zellbiologie.uni-bayreuth.de/sequences.htm
The depositor mtGFP insert seq shows 5' HindIII and a 3' EcoRI, they are actually reversed in this construct, the cloning information displayed is correct.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYX142-mtGFP was a gift from Benedikt Westermann (Addgene plasmid # 45050 ; http://n2t.net/addgene:45050 ; RRID:Addgene_45050) -
For your References section:
Mitochondria-targeted green fluorescent proteins: convenient tools for the study of organelle biogenesis in Saccharomyces cerevisiae. Westermann B, Neupert W. Yeast. 2000 Nov;16(15):1421-7. 10.1002/1097-0061(200011)16:15<1421::AID-YEA624>3.0.CO;2-U PubMed 11054823
Map uploaded by the depositor.
