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Addgene

pYX122-mtGFP
(Plasmid #45048)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45048 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYX122
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mtGFP
  • Promoter TPI

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

additional information may be found at:
http://www.zellbiologie.uni-bayreuth.de/sequences.htm

The depositor mtGFP insert seq shows 5' HindIII and a 3' EcoRI, they are actually reversed in this construct, the cloning information displayed is correct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYX122-mtGFP was a gift from Benedikt Westermann (Addgene plasmid # 45048 ; http://n2t.net/addgene:45048 ; RRID:Addgene_45048)

  • For your References section:

    Mitochondria-targeted green fluorescent proteins: convenient tools for the study of organelle biogenesis in Saccharomyces cerevisiae. Westermann B, Neupert W. Yeast. 2000 Nov;16(15):1421-7. 10.1002/1097-0061(200011)16:15<1421::AID-YEA624>3.0.CO;2-U PubMed 11054823
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