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Addgene

pCDNA-FRT-FAK0
(Plasmid #45047)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45047 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA-5/FRT
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5002
  • Total vector size (bp) 9812
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Focal Adhesion Kinase
  • Alt name
    FAK
  • Species
    G. gallus (chicken), Synthetic
  • Insert Size (bp)
    4810
  • Promoter CMV
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • mCitrine (N terminal on insert)
    • mCerulean (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGHR
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Discrepancies between QC and full sequence are of no functional consequence

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA-FRT-FAK0 was a gift from Sivaraj Sivaramakrishnan (Addgene plasmid # 45047 ; http://n2t.net/addgene:45047 ; RRID:Addgene_45047)

  • For your References section:

    Visualizing and manipulating focal adhesion kinase regulation in live cells. Ritt M, Guan JL, Sivaramakrishnan S. J Biol Chem. 2013 Mar 29;288(13):8875-86. doi: 10.1074/jbc.M112.421164. Epub 2013 Feb 7. 10.1074/jbc.M112.421164 PubMed 23393139
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