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Addgene

PGEX-NEDD4
(Plasmid #45043)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45043 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-5X3
  • Backbone manufacturer
    Amersham Pharmacia
  • Backbone size w/o insert (bp) 4972
  • Total vector size (bp) 7672
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NEDD4-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2700
  • GenBank ID
    NM_006154.2
  • Entrez Gene
    NEDD4 (a.k.a. NEDD4-1, RPF1)
  • Tag / Fusion Protein
    • GST

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PGEX-NEDD4 was a gift from Shiaw-Yih Lin (Addgene plasmid # 45043 ; http://n2t.net/addgene:45043 ; RRID:Addgene_45043)

  • For your References section:

    Rak functions as a tumor suppressor by regulating PTEN protein stability and function. Yim EK, Peng G, Dai H, Hu R, Li K, Lu Y, Mills GB, Meric-Bernstam F, Hennessy BT, Craven RJ, Lin SY. Cancer Cell. 2009 Apr 7;15(4):304-14. doi: 10.1016/j.ccr.2009.02.012. 10.1016/j.ccr.2009.02.012 PubMed 19345329
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