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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45029 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHM-GWA
- Backbone size w/o insert (bp) 8236
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCsy4H29AS50C
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SpeciesPseudomonas aeruginosa
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Insert Size (bp)564
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MutationH29A mutation to abolish catalytic activity and Ser50Cys mutation to enable coupling to biotin
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GenBank ID115585590 ABJ11605
- Promoter T7
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Tag
/ Fusion Protein
- His6-MBP (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer MBF Forward
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Csy4H29AS50C was a gift from Jennifer Doudna (Addgene plasmid # 45029 ; http://n2t.net/addgene:45029 ; RRID:Addgene_45029) -
For your References section:
RNA-protein analysis using a conditional CRISPR nuclease. Lee HY, Haurwitz RE, Apffel A, Zhou K, Smart B, Wenger CD, Laderman S, Bruhn L, Doudna JA. Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):5416-21. doi: 10.1073/pnas.1302807110. Epub 2013 Mar 14. 10.1073/pnas.1302807110 PubMed 23493562