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Addgene

pTC62.6-dmd-1, full ORF, splice form 1
(Plasmid #45028)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45028 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJC53.2
  • Backbone size w/o insert (bp) 3240
  • Total vector size (bp) 4299
  • Vector type
    T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smed-dmd-1, full ORF, spice form 1
  • Species
    Schmidtea mediterranea
  • Insert Size (bp)
    1059
  • GenBank ID
    KC736555

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eam1105I (destroyed during cloning)
  • 3′ cloning site Eam1105I (destroyed during cloning)
  • 5′ sequencing primer Sp6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Addgene QC sequencing was unable to verify bp 1-14 of the depositor sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTC62.6-dmd-1, full ORF, splice form 1 was a gift from Phillip Newmark (Addgene plasmid # 45028 ; http://n2t.net/addgene:45028 ; RRID:Addgene_45028)

  • For your References section:

    A sex-specific transcription factor controls male identity in a simultaneous hermaphrodite. Chong T, Collins JJ 3rd, Brubacher JL, Zarkower D, Newmark PA. Nat Commun. 2013; 4:1814. doi: 10.1038/ncomms2811. 10.1038/ncomms2811 PubMed 23652002
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