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Addgene

CAG-NeuroD2iresGFP
(Plasmid #45026)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45026 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGIG
  • Backbone manufacturer
    Matsuda & Cepko
  • Backbone size w/o insert (bp) 6135
  • Total vector size (bp) 7287
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NeuroD2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1149
  • Entrez Gene
    Neurod2 (a.k.a. Ndrf, bHLHa1)
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCAG-F
  • 3′ sequencing primer IRES-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAG-NeuroD2iresGFP was a gift from Connie Cepko (Addgene plasmid # 45026 ; http://n2t.net/addgene:45026 ; RRID:Addgene_45026)

  • For your References section:

    NeuroD factors regulate cell fate and neurite stratification in the developing retina. Cherry TJ, Wang S, Bormuth I, Schwab M, Olson J, Cepko CL. J Neurosci. 2011 May 18;31(20):7365-79. doi: 10.1523/JNEUROSCI.2555-10.2011. 10.1523/JNEUROSCI.2555-10.2011 PubMed 21593321
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