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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45025 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAGIG
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Backbone manufacturerMatsuda & Cepko
- Backbone size w/o insert (bp) 6135
- Total vector size (bp) 7196
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNeuroD1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1073
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Entrez GeneNeurod1 (a.k.a. BETA2, BHF-1, Nd1, Neurod, bHLHa3)
- Promoter CAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pCAG-F
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CAG-NeuroD1iresGFP was a gift from Connie Cepko (Addgene plasmid # 45025 ; http://n2t.net/addgene:45025 ; RRID:Addgene_45025) -
For your References section:
NeuroD factors regulate cell fate and neurite stratification in the developing retina. Cherry TJ, Wang S, Bormuth I, Schwab M, Olson J, Cepko CL. J Neurosci. 2011 May 18;31(20):7365-79. doi: 10.1523/JNEUROSCI.2555-10.2011. 10.1523/JNEUROSCI.2555-10.2011 PubMed 21593321
Map uploaded by the depositor.
