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Hsp70-mplum
(Plasmid #45016)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45016 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST-R4-R3
  • Backbone manufacturer
    Life Technologies/Invitrogen
  • Total vector size (bp) 4511
  • Modifications to backbone
    The attB1 site resulting from MultiSite Gateway recombination was lost owing to further modification in order to insert the beta-globin intron between the Hspa1b promoter and the mPlum coding sequence.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mPlum
  • Species
    Discosoma sp.
  • Insert Size (bp)
    681
  • GenBank ID
    AY786537.1
  • Promoter mouse Hspa1b

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13 reverse
  • 3′ sequencing primer M13 forward (-20)
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original mPlum cDNA for constructing our plasmid was initially provided by Dr Roger Y.Tsien (UCSD), and use was subject to their MTA.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Wang L, Jackson WC, Steinbach PA, Tsien RY. Evolution of new nonantibody
proteins via iterative somatic hypermutation. Proc Natl Acad Sci U S A. 2004 Nov
30;101(48):16745-9.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Hsp70-mplum was a gift from Anna Planas (Addgene plasmid # 45016 ; http://n2t.net/addgene:45016 ; RRID:Addgene_45016)

  • For your References section:

    In vivo imaging of induction of heat-shock protein-70 gene expression with fluorescence reflectance imaging and intravital confocal microscopy following brain ischaemia in reporter mice. de la Rosa X, Santalucia T, Fortin PY, Purroy J, Calvo M, Salas-Perdomo A, Justicia C, Couillaud F, Planas AM. Eur J Nucl Med Mol Imaging. 2013 Feb;40(3):426-38. doi: 10.1007/s00259-012-2277-7. Epub 2012 Nov 8. 10.1007/s00259-012-2277-7 PubMed 23135322
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