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Addgene

3X FLAG-FUS-WT
(Plasmid #44985)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44985 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5100
  • Total vector size (bp) 6700
  • Modifications to backbone
    3X FLAG Tag inserted at the HindIII site
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FUS (Full length)
  • Alt name
    TLS
  • Alt name
    fused in sarcoma
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • GenBank ID
  • Entrez Gene
    FUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV
  • 3′ sequencing primer BGH
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3X FLAG-FUS-WT was a gift from Robin Reed (Addgene plasmid # 44985 ; http://n2t.net/addgene:44985 ; RRID:Addgene_44985)

  • For your References section:

    FUS-SMN protein interactions link the motor neuron diseases ALS and SMA. Yamazaki T, Chen S, Yu Y, Yan B, Haertlein TC, Carrasco MA, Tapia JC, Zhai B, Das R, Lalancette-Hebert M, Sharma A, Chandran S, Sullivan G, Nishimura AL, Shaw CE, Gygi SP, Shneider NA, Maniatis T, Reed R. Cell Rep. 2012 Oct 25;2(4):799-806. doi: 10.1016/j.celrep.2012.08.025. Epub 2012 Sep 27. 10.1016/j.celrep.2012.08.025 PubMed 23022481
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