Skip to main content
Addgene

pCIN4-EZOD
(Plasmid #44977)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44977 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCIN4
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ets2 DBD
  • Species
    M. musculus (mouse)
  • Mutation
    Ets2 DNA binding domain (AA 332-468) only = dominant negative
  • Entrez Gene
    Ets2 (a.k.a. Ets-2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • SV40 NLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer pCMV fwd
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIN4-EZOD was a gift from Robert Oshima (Addgene plasmid # 44977 ; http://n2t.net/addgene:44977 ; RRID:Addgene_44977)

  • For your References section:

    Oncogenic Neu/ErbB-2 increases ets, AP-1, and NF-kappaB-dependent gene expression, and inhibiting ets activation blocks Neu-mediated cellular transformation. Galang CK, Garcia-Ramirez J, Solski PA, Westwick JK, Der CJ, Neznanov NN, Oshima RG, Hauser CA. J Biol Chem. 1996 Apr 5;271(14):7992-8. 10.1074/jbc.271.14.7992 PubMed 8626480
Related items: