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Addgene

HA-DDX3
(Plasmid #44975)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44975 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1/Hygro
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DDX3
  • Alt name
    DDX3X
  • Alt name
    DBX
  • Alt name
    HLP2
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_001193416
  • Entrez Gene
    DDX3X (a.k.a. CAP-Rf, DBX, DDX14, DDX3, HLP2, MRX102, MRXSSB)
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA (C terminal on insert)
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)
  • 5′ sequencing primer CMV
  • 3′ sequencing primer BGH
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original plasmid containing the DDX3 gene was obtained from Arvind Patel. It was published in Owsianka and Patel Virology 1999: 257, 330.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-DDX3 was a gift from Robin Reed (Addgene plasmid # 44975 ; http://n2t.net/addgene:44975 ; RRID:Addgene_44975)

  • For your References section:

    Human DDX3 functions in translation and interacts with the translation initiation factor eIF3. Lee CS, Dias AP, Jedrychowski M, Patel AH, Hsu JL, Reed R. Nucleic Acids Res. 2008 Aug;36(14):4708-18. doi: 10.1093/nar/gkn454. Epub 2008 Jul 15. 10.1093/nar/gkn454 PubMed 18628297
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