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pCMV-BD-cop1at(335-675)
(Plasmid #44973)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44973 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-BD
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4251
  • Total vector size (bp) 5730
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BD-COP1
  • Alt name
    CONSTITUTIVE PHOTOMORPHOGENIC 1
  • Alt name
    E3 ubiquitin-protein ligase COP1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1479
  • Mutation
    deleted amino acids 1-334
  • GenBank ID
    NM_128855.3
  • Entrez Gene
    COP1 (a.k.a. AT2G32950, ARABIDOPSIS THALIANA CONSTITUTIVE PHOTOMORPHOGENIC 1, ATCOP1, CONSTITUTIVE PHOTOMORPHOGENIC 1, DEETIOLATED MUTANT 340, DET340, EMB168, EMBRYO DEFECTIVE 168, FUS1, FUSCA 1, T21L14.11, T21L14_11)
  • Promoter pCMV
  • Tag / Fusion Protein
    • GAL4 DNA-binding domain (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-BD-cop1at(335-675) was a gift from Thanos Halazonetis (Addgene plasmid # 44973 ; http://n2t.net/addgene:44973 ; RRID:Addgene_44973)

  • For your References section:

    Ultraviolet-B-mediated induction of protein-protein interactions in mammalian cells. Crefcoeur RP, Yin R, Ulm R, Halazonetis TD. Nat Commun. 2013;4:1779. doi: 10.1038/ncomms2800. 10.1038/ncomms2800 PubMed 23653191
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