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pSNV2 CHER NLS cop1at(335-675)
(Plasmid #44972)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44972 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSV2neo
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4285
  • Total vector size (bp) 6055
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry-NLS-COP1
  • Alt name
    CONSTITUTIVELY PHOTOMORPHOGENIC1
  • Alt name
    E3 ubiquitin-protein ligase COP1
  • Species
    A. thaliana (mustard weed)
  • Mutation
    deleted amino acids 1-334
  • GenBank ID
    NM_128855.3
  • Entrez Gene
    COP1 (a.k.a. AT2G32950, ARABIDOPSIS THALIANA CONSTITUTIVE PHOTOMORPHOGENIC 1, ATCOP1, CONSTITUTIVE PHOTOMORPHOGENIC 1, DEETIOLATED MUTANT 340, DET340, EMB168, EMBRYO DEFECTIVE 168, FUS1, FUSCA 1, T21L14.11, T21L14_11)
  • Promoter pCMV IE
  • Tags / Fusion Proteins
    • mCherry (N terminal on insert)
    • NLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer SV40pro-F
  • 3′ sequencing primer CTGCTCCCATTCATCAGT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSNV2 CHER NLS cop1at(335-675) was a gift from Thanos Halazonetis (Addgene plasmid # 44972 ; http://n2t.net/addgene:44972 ; RRID:Addgene_44972)

  • For your References section:

    Ultraviolet-B-mediated induction of protein-protein interactions in mammalian cells. Crefcoeur RP, Yin R, Ulm R, Halazonetis TD. Nat Commun. 2013;4:1779. doi: 10.1038/ncomms2800. 10.1038/ncomms2800 PubMed 23653191
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