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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 44968 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNICE
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepCMV m-sGFP11::NXN
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SpeciesSynthetic
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Insert Size (bp)1400
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer CMV_fwd_primer
- 3′ sequencing primer EBV_rev_primer (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
m-sGFP11::NXN was a gift from Joshua Sanes (Addgene plasmid # 44968 ; http://n2t.net/addgene:44968 ; RRID:Addgene_44968) -
For your References section:
Transgenic strategy for identifying synaptic connections in mice by fluorescence complementation (GRASP). Yamagata M, Sanes JR. Front Mol Neurosci. 2012;5:18. Epub 2012 Feb 16. 10.3389/fnmol.2012.00018 PubMed 22355283