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Addgene

m-sGFP1-10::NLG1
(Plasmid #44967)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44967 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNICE
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    m-sGFP1-10::Neuroligin1
  • Alt name
    mGRASP
  • Species
    Synthetic
  • Insert Size (bp)
    3100
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV_fwd_primer
  • 3′ sequencing primer EBV_rev_primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    m-sGFP1-10::NLG1 was a gift from Joshua Sanes (Addgene plasmid # 44967 ; http://n2t.net/addgene:44967 ; RRID:Addgene_44967)

  • For your References section:

    Transgenic strategy for identifying synaptic connections in mice by fluorescence complementation (GRASP). Yamagata M, Sanes JR. Front Mol Neurosci. 2012;5:18. Epub 2012 Feb 16. 10.3389/fnmol.2012.00018 PubMed 22355283
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