NL4-3 mCherry Luciferase
(Plasmid #44965)

• Purpose: HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.
• Depositing Lab: Warner Greene
• Publication: Lassen et al PLoS One. 2012;7(1):e30176. doi: 10.1371/journal.pone.0030176. Epub 2012 Jan 24.

Backbone

• Vector backbone: pNLENG1
• Backbone manufacturer: AIDS Research and Reference Reagent Program
• Total vector size (bp): 12424
• Vector type: Lentiviral
• Selectable markers: mCherry, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mCherry, Luciferase, IRES
• Alt name: HIV 1 reporter virus
• Species: Lentivirus, Human specific
• Mutation: S34C mutation in Nef
• Tags / Fusion Proteins:
  • mCherry with D212G (C terminal on insert)
  • Luciferase, IRES (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: from pNLENG1 5'
• 3′ sequencing primer: from pNLENG1 3'

Resource Information

• Supplemental Documents:
  • NL43mCHLucSEQnotated
• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: pNL4-3 obtained from the AIDS Research and Reference Reagent Program pSicoRMS2 from Matt Spindler and Bruce Conklin, Gladstone Institute of Cardiovascular Disease
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the D212G mutation in mCherry has no effect on the function of this plasmid.