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PurposeYeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with Kan selection
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44943 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFA6a
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Vector typeYeast Expression
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Selectable markersKanamycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameyoLSS-mKate2
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Alt nameYeast Optimized LSS-mKate2
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SpeciesSynthetic
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.
These plasmids were generated with funding from an NIGMS Systems Biology Center grant, number P50 GM081879. Please acknowledge this grant if you use these plasmids.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-link-yoLSS-mKate2-Kan was a gift from Wendell Lim & Kurt Thorn (Addgene plasmid # 44943 ; http://n2t.net/addgene:44943 ; RRID:Addgene_44943) -
For your References section:
Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae. Lee S, Lim WA, Thorn KS. PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902. Print 2013. 10.1371/journal.pone.0067902 PubMed 23844123
