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pET15b-At 14-3-3 upsilon
(Plasmid #44932)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44932 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 6500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    14-3-3 upsilon
  • Alt name
    GRF5
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    807
  • GenBank ID
    NM_121610.3
  • Entrez Gene
    GRF5 (a.k.a. AT5G16050, F1N13.190, F1N13_190, GF14 UPSILON, GF14 UPSILON CHAIN, general regulatory factor 5)
  • Promoter T7
  • Tag / Fusion Protein
    • His tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b-At 14-3-3 upsilon was a gift from Robert Ferl (Addgene plasmid # 44932 ; http://n2t.net/addgene:44932 ; RRID:Addgene_44932)

  • For your References section:

    The heterologous interactions among plant 14-3-3 proteins and identification of regions that are important for dimerization. Wu K, Lu G, Sehnke P, Ferl RJ. Arch Biochem Biophys. 1997 Mar 1;339(1):2-8. 10.1006/abbi.1996.9841 PubMed 9056226
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