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Addgene

pET15b-At 14-3-3 omicron
(Plasmid #44926)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44926 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 6500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    14-3-3 omicron
  • Alt name
    GRF11
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    759
  • GenBank ID
    NM_001084180.1
  • Entrez Gene
    GRF11 (a.k.a. AT1G34760, 14-3-3 PROTEIN GF14OMICRON, F11O6.13, GF14 OMICRON, RHS5, ROOT HAIR SPECIFIC 5, general regulatory factor 11)
  • Promoter T7
  • Tag / Fusion Protein
    • His tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NDEI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b-At 14-3-3 omicron was a gift from Robert Ferl (Addgene plasmid # 44926 ; http://n2t.net/addgene:44926 ; RRID:Addgene_44926)
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