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Addgene

Mumps V-FLAG
(Plasmid #44908)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44908 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEF-FLAG
  • Backbone size w/o insert (bp) 5358
  • Total vector size (bp) 6000

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mumps V
  • Insert Size (bp)
    675
  • GenBank ID
    P30927.1
  • Entrez Gene
    V/P (a.k.a. MuVgp2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer EF1a-F
  • 3′ sequencing primer ??
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The discrepancies between the depositor's sequence and the Addgene QC sequence should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mumps V-FLAG was a gift from Curt Horvath (Addgene plasmid # 44908 ; http://n2t.net/addgene:44908 ; RRID:Addgene_44908)
  • For your References section:

    STAT3 ubiquitylation and degradation by mumps virus suppress cytokine and oncogene signaling. Ulane CM, Rodriguez JJ, Parisien JP, Horvath CM. J Virol. 2003 Jun;77(11):6385-93. 10.1128/JVI.77.11.6385-6393.2003 PubMed 12743296