PIV5-V-FLAG
(Plasmid
#44904)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44904 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEF
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5358
- Total vector size (bp) 6000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePIV5
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Alt nameSV5
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GenBank IDYP_138513.1
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Entrez GeneV/P (a.k.a. SPV5gp2)
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer EF1a-F
- 3′ sequencing primer ?? (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PIV5-V-FLAG was a gift from Curt Horvath (Addgene plasmid # 44904 ; http://n2t.net/addgene:44904 ; RRID:Addgene_44904) -
For your References section:
Selective STAT protein degradation induced by paramyxoviruses requires both STAT1 and STAT2 but is independent of alpha/beta interferon signal transduction. Parisien JP, Lau JF, Rodriguez JJ, Ulane CM, Horvath CM. J Virol. 2002 May;76(9):4190-8. 10.1128/JVI.76.9.4190-4198.2002 PubMed 11932384