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Addgene

pFA6a-link-yomCherry-CaURA3 Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 44876 with HindIII-HF and EcoRI-HF.

We expected and saw the following banding patterns:

HindIII-HF single digest: 1 band: 4.9kb

HindIII-HF / EcoRI-HF double digest: 3 bands: 2.4, 1.5, and 0.9kb