pFA6a-link-yomTagBFP-SpHis5
(Plasmid #44838)

• Purpose: Yeast tagging vector to create gene fusion with yeast optimized mTagBFP with SpHis5 selection
• Depositing Labs: Wendell Lim, Kurt Thorn
• Publication: Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902. Print 2013.

Backbone

• Vector backbone: pFA6a
• Vector type: Yeast Expression
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: yomTagBFP
• Alt name: Yeast Optimized mTagBFP
• Species: Synthetic

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.

These plasmids were generated with funding from an NIGMS Systems Biology Center grant, number P50 GM081879. Please acknowledge this grant if you use these plasmids.