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pFA6a-link-yoSuperfolderGFP-SpHis5
(Plasmid #44837)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44837 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    yoSuperfolderGFP
  • Alt name
    Yeast Optimized SuperfolderGFP
  • Species
    Synthetic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.

These plasmids were generated with funding from an NIGMS Systems Biology Center grant, number P50 GM081879. Please acknowledge this grant if you use these plasmids.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-link-yoSuperfolderGFP-SpHis5 was a gift from Wendell Lim & Kurt Thorn (Addgene plasmid # 44837 ; http://n2t.net/addgene:44837 ; RRID:Addgene_44837)

  • For your References section:

    Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae. Lee S, Lim WA, Thorn KS. PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902. Print 2013. 10.1371/journal.pone.0067902 PubMed 23844123
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